hitrap sp hp cation exchange chromatography column Search Results


perfusion chromatography cation exchange column (poros 20 sp  (PerSeptive Biosystems Inc)
90
PerSeptive Biosystems Inc perfusion chromatography cation exchange column (poros 20 sp
Perfusion Chromatography Cation Exchange Column (Poros 20 Sp, supplied by PerSeptive Biosystems Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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fast flow sp sepharose cation chromatography  (Cytiva Europe)
96
Cytiva Europe fast flow sp sepharose cation chromatography
E2A-AD1 binds the TAZ2 domain of CBP/p300. A, 15% SDS-PAGE analysis of the ability of various purified His6-GB1-E2A fragments immobilized on <t>IgG-agarose</t> to pull down purified recombinant KIX, TAZ1, and TAZ2 domains of p300. The left lanes of each panel represent controls illustrating the migration of the recombinant isolated p300 domains. B, quantitative analysis of the His6-GB1-E2A pulldowns presented in A. Significance between pulldown protein levels of TAZ1, TAZ2, and KIX by GB1 (negative control) and GB1-E2A constructs is indicated by brackets. Error bars, S.D. (**, p < 0.01; NS, not significant).
Fast Flow Sp Sepharose Cation Chromatography, supplied by Cytiva Europe, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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fast flow sp sepharose cation chromatography - by Bioz Stars, 2026-10
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cation exchange ciex chromatography column  (Cytiva Europe)
98
Cytiva Europe cation exchange ciex chromatography column
E2A-AD1 binds the TAZ2 domain of CBP/p300. A, 15% SDS-PAGE analysis of the ability of various purified His6-GB1-E2A fragments immobilized on <t>IgG-agarose</t> to pull down purified recombinant KIX, TAZ1, and TAZ2 domains of p300. The left lanes of each panel represent controls illustrating the migration of the recombinant isolated p300 domains. B, quantitative analysis of the His6-GB1-E2A pulldowns presented in A. Significance between pulldown protein levels of TAZ1, TAZ2, and KIX by GB1 (negative control) and GB1-E2A constructs is indicated by brackets. Error bars, S.D. (**, p < 0.01; NS, not significant).
Cation Exchange Ciex Chromatography Column, supplied by Cytiva Europe, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cation exchange ciex chromatography column - by Bioz Stars, 2026-10
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hitrap sp hp column  (Cytiva Europe)
96
Cytiva Europe hitrap sp hp column
E2A-AD1 binds the TAZ2 domain of CBP/p300. A, 15% SDS-PAGE analysis of the ability of various purified His6-GB1-E2A fragments immobilized on <t>IgG-agarose</t> to pull down purified recombinant KIX, TAZ1, and TAZ2 domains of p300. The left lanes of each panel represent controls illustrating the migration of the recombinant isolated p300 domains. B, quantitative analysis of the His6-GB1-E2A pulldowns presented in A. Significance between pulldown protein levels of TAZ1, TAZ2, and KIX by GB1 (negative control) and GB1-E2A constructs is indicated by brackets. Error bars, S.D. (**, p < 0.01; NS, not significant).
Hitrap Sp Hp Column, supplied by Cytiva Europe, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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hitrap sp hp column - by Bioz Stars, 2026-10
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cation exchange chromatography  (Cytiva Europe)
96
Cytiva Europe cation exchange chromatography
E2A-AD1 binds the TAZ2 domain of CBP/p300. A, 15% SDS-PAGE analysis of the ability of various purified His6-GB1-E2A fragments immobilized on <t>IgG-agarose</t> to pull down purified recombinant KIX, TAZ1, and TAZ2 domains of p300. The left lanes of each panel represent controls illustrating the migration of the recombinant isolated p300 domains. B, quantitative analysis of the His6-GB1-E2A pulldowns presented in A. Significance between pulldown protein levels of TAZ1, TAZ2, and KIX by GB1 (negative control) and GB1-E2A constructs is indicated by brackets. Error bars, S.D. (**, p < 0.01; NS, not significant).
Cation Exchange Chromatography, supplied by Cytiva Europe, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/hitrap+sp+hp+cation+exchange+chromatography+column/HiTrap+SP+FF/pmc04945660-349-6-15
Average 96 stars, based on 1 article reviews
cation exchange chromatography - by Bioz Stars, 2026-10
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sp sepharose cation exchange column  (Cytiva Europe)
96
Cytiva Europe sp sepharose cation exchange column
E2A-AD1 binds the TAZ2 domain of CBP/p300. A, 15% SDS-PAGE analysis of the ability of various purified His6-GB1-E2A fragments immobilized on <t>IgG-agarose</t> to pull down purified recombinant KIX, TAZ1, and TAZ2 domains of p300. The left lanes of each panel represent controls illustrating the migration of the recombinant isolated p300 domains. B, quantitative analysis of the His6-GB1-E2A pulldowns presented in A. Significance between pulldown protein levels of TAZ1, TAZ2, and KIX by GB1 (negative control) and GB1-E2A constructs is indicated by brackets. Error bars, S.D. (**, p < 0.01; NS, not significant).
Sp Sepharose Cation Exchange Column, supplied by Cytiva Europe, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/hitrap+sp+hp+cation+exchange+chromatography+column/Sepharose+4B/pmc03154623-357-22-26
Average 96 stars, based on 1 article reviews
sp sepharose cation exchange column - by Bioz Stars, 2026-10
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hitrap sp hp  (Cytiva Europe)
97
Cytiva Europe hitrap sp hp
E2A-AD1 binds the TAZ2 domain of CBP/p300. A, 15% SDS-PAGE analysis of the ability of various purified His6-GB1-E2A fragments immobilized on <t>IgG-agarose</t> to pull down purified recombinant KIX, TAZ1, and TAZ2 domains of p300. The left lanes of each panel represent controls illustrating the migration of the recombinant isolated p300 domains. B, quantitative analysis of the His6-GB1-E2A pulldowns presented in A. Significance between pulldown protein levels of TAZ1, TAZ2, and KIX by GB1 (negative control) and GB1-E2A constructs is indicated by brackets. Error bars, S.D. (**, p < 0.01; NS, not significant).
Hitrap Sp Hp, supplied by Cytiva Europe, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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strong cation exchange chromatography column uni sp 50 xs  (Suzhou Nanomicro)
90
Suzhou Nanomicro strong cation exchange chromatography column uni sp 50 xs
E2A-AD1 binds the TAZ2 domain of CBP/p300. A, 15% SDS-PAGE analysis of the ability of various purified His6-GB1-E2A fragments immobilized on <t>IgG-agarose</t> to pull down purified recombinant KIX, TAZ1, and TAZ2 domains of p300. The left lanes of each panel represent controls illustrating the migration of the recombinant isolated p300 domains. B, quantitative analysis of the His6-GB1-E2A pulldowns presented in A. Significance between pulldown protein levels of TAZ1, TAZ2, and KIX by GB1 (negative control) and GB1-E2A constructs is indicated by brackets. Error bars, S.D. (**, p < 0.01; NS, not significant).
Strong Cation Exchange Chromatography Column Uni Sp 50 Xs, supplied by Suzhou Nanomicro, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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sp sepharose  (Danaher Inc)
94
Danaher Inc sp sepharose
E2A-AD1 binds the TAZ2 domain of CBP/p300. A, 15% SDS-PAGE analysis of the ability of various purified His6-GB1-E2A fragments immobilized on <t>IgG-agarose</t> to pull down purified recombinant KIX, TAZ1, and TAZ2 domains of p300. The left lanes of each panel represent controls illustrating the migration of the recombinant isolated p300 domains. B, quantitative analysis of the His6-GB1-E2A pulldowns presented in A. Significance between pulldown protein levels of TAZ1, TAZ2, and KIX by GB1 (negative control) and GB1-E2A constructs is indicated by brackets. Error bars, S.D. (**, p < 0.01; NS, not significant).
Sp Sepharose, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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hitrap ff chromatography columns  (Cytiva Europe)
96
Cytiva Europe hitrap ff chromatography columns
E2A-AD1 binds the TAZ2 domain of CBP/p300. A, 15% SDS-PAGE analysis of the ability of various purified His6-GB1-E2A fragments immobilized on <t>IgG-agarose</t> to pull down purified recombinant KIX, TAZ1, and TAZ2 domains of p300. The left lanes of each panel represent controls illustrating the migration of the recombinant isolated p300 domains. B, quantitative analysis of the His6-GB1-E2A pulldowns presented in A. Significance between pulldown protein levels of TAZ1, TAZ2, and KIX by GB1 (negative control) and GB1-E2A constructs is indicated by brackets. Error bars, S.D. (**, p < 0.01; NS, not significant).
Hitrap Ff Chromatography Columns, supplied by Cytiva Europe, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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hitrap ff chromatography columns - by Bioz Stars, 2026-10
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cation exchange chromatography  (Danaher Inc)
94
Danaher Inc cation exchange chromatography
E2A-AD1 binds the TAZ2 domain of CBP/p300. A, 15% SDS-PAGE analysis of the ability of various purified His6-GB1-E2A fragments immobilized on <t>IgG-agarose</t> to pull down purified recombinant KIX, TAZ1, and TAZ2 domains of p300. The left lanes of each panel represent controls illustrating the migration of the recombinant isolated p300 domains. B, quantitative analysis of the His6-GB1-E2A pulldowns presented in A. Significance between pulldown protein levels of TAZ1, TAZ2, and KIX by GB1 (negative control) and GB1-E2A constructs is indicated by brackets. Error bars, S.D. (**, p < 0.01; NS, not significant).
Cation Exchange Chromatography, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


E2A-AD1 binds the TAZ2 domain of CBP/p300. A, 15% SDS-PAGE analysis of the ability of various purified His6-GB1-E2A fragments immobilized on IgG-agarose to pull down purified recombinant KIX, TAZ1, and TAZ2 domains of p300. The left lanes of each panel represent controls illustrating the migration of the recombinant isolated p300 domains. B, quantitative analysis of the His6-GB1-E2A pulldowns presented in A. Significance between pulldown protein levels of TAZ1, TAZ2, and KIX by GB1 (negative control) and GB1-E2A constructs is indicated by brackets. Error bars, S.D. (**, p < 0.01; NS, not significant).

Journal: The Journal of Biological Chemistry

Article Title: Structural insights into TAZ2 domain–mediated CBP/p300 recruitment by transactivation domain 1 of the lymphopoietic transcription factor E2A

doi: 10.1074/jbc.RA119.011078

Figure Lengend Snippet: E2A-AD1 binds the TAZ2 domain of CBP/p300. A, 15% SDS-PAGE analysis of the ability of various purified His6-GB1-E2A fragments immobilized on IgG-agarose to pull down purified recombinant KIX, TAZ1, and TAZ2 domains of p300. The left lanes of each panel represent controls illustrating the migration of the recombinant isolated p300 domains. B, quantitative analysis of the His6-GB1-E2A pulldowns presented in A. Significance between pulldown protein levels of TAZ1, TAZ2, and KIX by GB1 (negative control) and GB1-E2A constructs is indicated by brackets. Error bars, S.D. (**, p < 0.01; NS, not significant).

Article Snippet: The cleaved, refolded TAZ1 or TAZ2 constructs were separated from the His 6 -GB1 fragments via Fast Flow SP Sepharose cation chromatography (GE Healthcare) using buffer A as a wash buffer and eluted with buffer A containing 500 m m NaCl.

Techniques: SDS Page, Purification, Recombinant, Migration, Isolation, Negative Control, Construct

Mutation of AD1 or AD2 of E2A(1–483) reduces its ability to pull down CBP. A, immunoblotting of comparative pulldowns of FLAG-CBP by purified His6-GB1-E2A(1–483) constructs immobilized on IgG-agarose. B, relative quantitative analysis of the Western blots performed in triplicate. ImageJ was used to measure the pixel density for each lane. The y axis values represent the ratio of FLAG-CBP pulldown by His6-GB1-E2A(1–483) mutants to FLAG-CBP pulldown by His6-GB1-E2A WT. Error bars, S.D. (n = 3; **, p < 0.0005; ***, p < 0.0001).

Journal: The Journal of Biological Chemistry

Article Title: Structural insights into TAZ2 domain–mediated CBP/p300 recruitment by transactivation domain 1 of the lymphopoietic transcription factor E2A

doi: 10.1074/jbc.RA119.011078

Figure Lengend Snippet: Mutation of AD1 or AD2 of E2A(1–483) reduces its ability to pull down CBP. A, immunoblotting of comparative pulldowns of FLAG-CBP by purified His6-GB1-E2A(1–483) constructs immobilized on IgG-agarose. B, relative quantitative analysis of the Western blots performed in triplicate. ImageJ was used to measure the pixel density for each lane. The y axis values represent the ratio of FLAG-CBP pulldown by His6-GB1-E2A(1–483) mutants to FLAG-CBP pulldown by His6-GB1-E2A WT. Error bars, S.D. (n = 3; **, p < 0.0005; ***, p < 0.0001).

Article Snippet: The cleaved, refolded TAZ1 or TAZ2 constructs were separated from the His 6 -GB1 fragments via Fast Flow SP Sepharose cation chromatography (GE Healthcare) using buffer A as a wash buffer and eluted with buffer A containing 500 m m NaCl.

Techniques: Mutagenesis, Western Blot, Purification, Construct